Signalling

Part:BBa_K3804010

Designed by: iGEM21_UTokyo   Group: iGEM21_UTokyo   (2021-09-26)


Reporter for quorum sensing systems in yeast with LasR

We created this part with reference to BBa_K1024002in order to signal when Pseudomonas aeruginosa exists. The part is composed of pTEF, Flag, VP16, NLS, LasR, ADH1 terminator, pLas, Cyc100mini, mCherry and CYC1terminator. We improved BBa_K1024002 by adding ADH1 terminator(BBa_J63002) and CYC1terminator(BBa_K1462070) and changed LuxR to LasR, pLux to pLas. LasR is homolog of LusR. The LasR gene is constitutively expressed. On the other hand, activation of pLas occurs only when sufficient amounts of AHL exist in the environment. When pLas was activated, mCherry is expressed and lets us know the existence of AHL.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 2567
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1580
    Illegal BamHI site found at 2738
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1928
    Illegal AgeI site found at 2125
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 506
    Illegal BsaI site found at 893
    Illegal BsaI site found at 1280
    Illegal BsaI.rc site found at 547
    Illegal BsaI.rc site found at 934
    Illegal BsaI.rc site found at 1321


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Parameters
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